I usually do not like to write about photoaffinity labeling reagents because of their indiscriminate nature. But this publication from the lab of Dr. Christina Woo in Harward. They were able to develop Cyclobutane Diazirine tags that can label Carboxylic Acids on the protein with variety of functionalities with pretty respectable efficiency (5-25%)! They used […]
Better site specificity for Photoaffinity Labeling Read More »
This method comes to us from Switzerland’s very own Ecole Polytechnique Fédérale de Lausanne, group of Dr. Waser. They decided to functionalize Tyrosine using Hypervalent Iodine compounds. To be precise, they used ethynylbenziodoxolones (EBX). It was pretty selective to Tyrosine, compared to other nucleophilic residues, and was slightly reactive towards cysteine. But, if there is
Very unusual way to add Azide to Tyrosine Read More »
This research comes to us from very reputable group of Dr. Yiyi Weng at Zhejiang University of Technology. This paper shows how to turn any Tryptophan into strange tertiary azide heterocycle with about 87% efficiency. Could be useful if no other functionalization methods are available. I am not sure how reactive this azide will
Very unusual method of turning Tryptophan into click-reactive handle Read More »
Here comes new modification of the Alkyne. Is this the new click chemistry? Through a modified Kinugasa reaction, a novel method of amidation on terminal oligo alkyne conjugates by copper-promoted oxidation with nitrones has been developed. Unprotected bifunctional carboxylic acid–amine reagents can be transformed directly to the respective amide products under these edited Kinugasa reaction
Very Interesting Alkyne Reaction Read More »